Лиофилизация мужских гамет сельскохозяйственных животных и птиц: достижения, проблемы, перспективы
https://doi.org/10.25687/3034-493X.2025.2.1.003
Аннотация
В настоящее время самым распространенным способом для длительного хранения репродуктивных клеток сельскохозяйственных животных и птиц является технология криоконсервации, которая предполагает хранение образцов в условиях сверхнизких температур. Однако значительные затраты на сохранность образцов и их транспортировку снижают показатели экономической эффективности метода. Лиофилизированная сперма может храниться при положительных температурах, и не требует для этого специального оборудования. На данный момент известно, что протоколы лиофилизации позволяют сохранять целостность хроматина сперматозоидов и факторы, детерминирующие активацию ооцитов у грызунов и млекопитающих, а метод интрацитоплазматической инъекции сперматозоида в яйцеклетку (ИКСИ) открывает возможность и перспективы использования «делиофилизированных» мужских гамет в репродуктивных технологиях. В статье представлен обзор литературы по проблеме сохранения спермы сельскохозяйственных животных в состоянии ангидробиоза. Описана история появления метода сублимационной сушки биологических образцов, проблемы предшествующих технологических этапов у разных видов сельскохозяйственных животных, резюмируются достижения и обозначаются тренды технологии лиофилизации мужских гамет животных
Об авторах
А. А. Курочкин
ВНИИГРЖ — филиал ФГБНУ ФИЦ ВИЖ им. Л.К. Эрнста
Россия
Россия
Санкт-Петербург
А. О. Притужалова
ВНИИГРЖ — филиал ФГБНУ ФИЦ ВИЖ им. Л.К. Эрнста
Россия
Россия
Санкт-Петербург
Т. И. Кузьмина
ВНИИГРЖ — филиал ФГБНУ ФИЦ ВИЖ им. Л.К. Эрнста
Россия
Россия
Санкт-Петербург
О. И. Станишевская
ВНИИГРЖ — филиал ФГБНУ ФИЦ ВИЖ им. Л.К. Эрнста
Россия
Россия
Санкт-Петербург
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Рецензия
Для цитирования:
Курочкин А.А., Притужалова А.О., Кузьмина Т.И., Станишевская О.И. Лиофилизация мужских гамет сельскохозяйственных животных и птиц: достижения, проблемы, перспективы. Успехи наук о животных. 2025;(1):33-52. https://doi.org/10.25687/3034-493X.2025.2.1.003
For citation:
Kurochkin A.A., Prituzhalova A.O., Kuzmina T.I., Stanishevskaya O.I. Lyophilization of male gametes of agricultural animals and birds: achievements, problems, prospects. Ernst Journal of Animal Science. 2025;(1):33-52. (In Russ.) https://doi.org/10.25687/3034-493X.2025.2.1.003
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